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Hunting Novel Viruses in a Lab Course

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Last Fall the Biology department mounted a new course (BIOL 152B),  the Virus Hunter Lab. This course combines practical experience in the lab with computer based approaches in bioinformatics. Students in the class isolated a type of virus that infects bacteria called a bacteriophage. In the wet lab, they learned basic microbiology techniques for isolating the viruses and basic molecular biology techniques for extracting the DNA of the viral genomes. With the biological material in hand the class prepared next generation sequencing libraries. The students assembled and annotated the complete genome of two previously unknown bacteriophage using next generation sequencing data from the samples they prepared. To find out more about it you can read their paper. Grad students Meghan Harris (MCB), their TA, and Tereasa Ho (Biotechnology) along with the inaugural group of undergraduate students are all authors on a paper recently published in the journal Microbiology Resource Announcements (MRA).

Harris MT, Ho TC, Fruchtman H, Garin ME, Kubatin V, Lu T, Xue L, Marr MT. Complete Genome Sequences of Two Vibrio natriegens Bacteriophages. Microbiology Resource Announcements. 2020;9(45).

Electron microscope image of the novel bacteriophage (VH2), photo by Jesse Cochrane


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